Device

Part:BBa_K4784002

Designed by: Airi Jitsushita   Group: iGEM23_Qdai   (2023-10-11)


Pdr12-AlaDH Synthesize pyruvate

This part of the plasmid is located downstream of the lac promoter. Pdr12, a membrane protein that effluxes pyruvate, is induced by IPTG. AlaDH, which converts alanine to pyruvate, is expressed downstream of the constitutive promoter. The incorporated alanine is converted to pyruvate, and pyruvate is transported out of the cell.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 576
    Illegal PstI site found at 1441
    Illegal PstI site found at 1789
    Illegal PstI site found at 3526
    Illegal PstI site found at 4036
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4706
    Illegal NheI site found at 4729
    Illegal PstI site found at 576
    Illegal PstI site found at 1441
    Illegal PstI site found at 1789
    Illegal PstI site found at 3526
    Illegal PstI site found at 4036
    Illegal NotI site found at 3869
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 135
    Illegal BamHI site found at 2066
    Illegal BamHI site found at 4179
    Illegal BamHI site found at 5018
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 576
    Illegal PstI site found at 1441
    Illegal PstI site found at 1789
    Illegal PstI site found at 3526
    Illegal PstI site found at 4036
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 576
    Illegal PstI site found at 1441
    Illegal PstI site found at 1789
    Illegal PstI site found at 3526
    Illegal PstI site found at 4036
    Illegal NgoMIV site found at 571
    Illegal NgoMIV site found at 1084
    Illegal NgoMIV site found at 1882
    Illegal NgoMIV site found at 2751
    Illegal NgoMIV site found at 3328
    Illegal NgoMIV site found at 3855
    Illegal NgoMIV site found at 4056
    Illegal AgeI site found at 2379
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None